第1页参见附件。
摘 要：目的：构建小鼠生精相关基因Vad1.2表达载体并进行鉴定。方法： 将小鼠睾丸组织中Vad1.2转录本进行RT-PCR扩增，通过DNA重组技术将其插入克隆载体pET15b中，将表达载体转化入大肠杆菌BL21(DE3)RIL感受态细胞，提取重组质粒并通过酶切及DNA测序进行鉴定。结果：随机挑取的克隆经双酶切鉴定，证实Vad1.2表达载体构建成功，插入基因序列正确。结论： 成功构建了小鼠Vad1.2基因表达载体，为Vad1.2基因的进一步研究奠定了实验基础。
关键词：精子发生；Vad1.2；小鼠
Abstract：Objective： To construct and characterize the Vad1.2 expression vector related to spermatogenesis in mice. Methods： Vad1.2 transcript was amplified from mouse testis by RT-PCR. The amplified product was inserted into pET15b vector. Then the Vad1.2 over-expression vector was transformed into E. coli BL21 (DE3) RIL competent cells and identified by endonuclease digestion and DNA sequencing ......
http://www.100md.com/html/paper/1674-9855B/2014/23/72.htm
您现在查看是摘要介绍页，详见PDF附件。
