【摘要】目的：用高效液相色谱法测定注射用肌苷的含量。方法：采用Kromasil C18柱(5μm 4.6mm×250mm)，以甲醇-水(10∶90)作流动相，在248nm紫外波长检测。结果：肌苷在4.23～63.48μg/ml范围内有良好的线性关系(r=0.9999)。低、中、高3个浓度的平均回收率(n=2)分别为101.4%、101.8%、101.1%，RSD为0.46%。结论：该法简便、快速、准确，可作为测定注射用肌苷中肌苷的含量。

 【关键词】注射用肌苷；高效液相色谱法；含量测定

 【Abstract】ObjectiveA high-performance liquid chromatography (HPLC) was established for the determination of inosine contents in injection.MethodsImpurity in the inosine injection could be separated on Kromasil C18 colnmn (5μm 4.6mm×250mm) using methanol-water (10:90) as mobile phase and UV detector at 248nm.ResultsThe linear range was 4.23～63.48μg/ml(r=0.9999).Average recovery in the lower,moderate and higher inosine concentration groups was respectively 101.4%,101.8% and 101.1%(n=2) with RSD of 0.46%.ConclusionThe method is simple,rapid and accurate.

 【Key words】Inosine injection;HPLC;Concentration determination

 【中图分类号】R927.2 【文献标识码】B 【文章编号】1006-1959(2009)06-0244-01

 肌苷为微生物发酵制得的辅酶类药物 ......
 百拇医药网 http://www.100md.com/html/paper/1006-1959/2009/06/224.htm
您现在查看是摘要页，全文长 4132 字符。