【摘要】 目的 探討使用氧化偶氮甲烷(AOM)及AOM联合葡聚糖硫酸钠(DSS)中不同药物剂量、给药周期及暴露时间对C57BL/6J小鼠建立结肠癌诱导模型效果的影响。方法 分别予低、中、高浓度AOM(10、15、20 mg/kg，每周1次，共4周，每组24只，AOM对照组24只)或AOM(10 mg/kg)联合DSS(一、二、三循环，小鼠数量分别为50、41、29只，DSS对照组40只)干预，对给药后不同时间点处死的小鼠结直肠进行大体与组织病理学评估。结果 单独使用AOM可使小鼠结肠产生异常隐窝灶(ACF)，随着AOM药物浓度和(或)暴露时间增加，ACF数量显著增加(P均, 百拇医药
 Comparative analysis between application of AOM and AOM/DSS to establish C57BL/6J mouse models with colonic carcinogenesis Zeng Yiwen， Li Jieyao， Tian Kuangyi， Yu Tao， Xu Jihao， Chen Qikui Department of Gastroenterology， Sun Yatsen Memorial Hospital， Sun Yatsen University， Guangzhou 510120， China

 Corresponding author，Tian Kuangyi， Email： sttupac@yahoocom

 【Abstract】 Objective To evaluate the effect of drug dose， cycle of drug administration and exposure time between azoxymethane (AOM) and AOM combined with dextran sodium sulfate(DSS) in the establishment of C57BL/6J mouse models with colonic carcinogenesis Methods Mice were treated with subcutaneous administration of 10， 15 or 20 mg/kg AOM once a week for 4 weeks (n=24 for each dose group and control group)， or with 10 mg/kg AOM followed by one， two， or three cycles of 2% DSS administration (n=50， 41 and 29 for each cycle group， and n=24 for control group) The colon tissues were collected at different time points after drug administration for gross and histopathological examinations Results Single use of AOM could yield aberrant crypt foci (ACF) The quantity of ACF was significantly increased along with the increasing AOM concentration and/or exposure time (all P, 百拇医药(曾一文 黎洁瑶 田旷怡 于涛 许稷豪 陈其奎) 第 1 2 3 4 页 下一页

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 百拇医药网 http://www.100md.com/html/paper/0253-9802/2018/10/06.htm