【摘要】 目的 探討Toll样受体-4(TLR4)沉默对高糖诱导胰岛微血管内皮细胞损伤的干预作用及机制。方法 利用 siRNA 沉默MS-1细胞中TLR4的表达，实时荧光定量聚合酶链反应(qRT-PCR)检测TLR4 mRNA的表达，CCK-8 法检测细胞增殖，比色法测定一氧化氮水平，ELISA检测内皮素-1水平，羟胺法检测超氧化物歧化酶(SOD)活力，硫代巴比妥酸(TBA)法检测丙二醛水平，蛋白免疫印迹法检测TLR4、髓样分化因子88(MyD88)和核转录因子(NF)-κBp65蛋白的表达。将高糖处理的细胞分为高糖组及siRNA处理组(再分为siRNA-TLR4组及NC-TLR4组)进行上述指标的比较。结果 与高糖组比较，siRNA-TLR4组TLR4 mRNA的相对表达水平降低，细胞的增殖能力受抑制(P均, http://www.100md.com
 【关键词】 Toll样受体-4;高糖;胰岛;微血管内皮细胞

 【Abstract】 Objective To evaluate the effect and mechanism of TLR4 silencing on high glucose-induced islet microvascular endothelial cell injury. Methods The expression of TLR4 in the MS-1 cells was silenced by siRNA. The expression of TLR4 mRNA was detected by qRT-PCR. The cell proliferation was measured by CCK-8 assay. The nitric oxode(NO) level was determined by colorimetry. The endothelin (ET)-1 level was measured by ELISA. The superoxide dismutase (SOD) activity was detected by hydroxylamine method. The malondialdehyde (MDA) level was detected by thiobarbituric acid (TBA) method. The expression levels of TLR4， MyD88 and NF-κBp65 proteins were detected by Western blot. The cells treated with high glucose were divided into the high glucose and isRNA silencing groups (further divided into siRNA-TLR4 and NC-TLR4 groups). Relevant parameters were statistically compared among different groups. Results Compared with the high glucose group， the relative expression level of TLR4 mRNA was significantly down-regulated， and the proliferation ability of the cells was inhibited in the siRNA-TLR4 group (both P , http://www.100md.com(林雪波) 第 1 2 3 4 页 下一页
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