[摘要] 目的 通过分析超声背向散射积分(IBS)参数的变化评价苯那普利对自发性高血压大鼠(SHR)左室心肌纤维化的影响。方法 14只雄性SHR随机分成模型对照组(SHR组，n=7)和药物干预组(SB组,n=7),另外选择7只雄性Wistar京都大鼠(WKY)作为正常对照组(WKY组)。SB组给予苯那普利灌胃(10 mg kg-1 d-1)，WKY及SHR组给予等量蒸馏水灌胃。每周秤体质量，调节用药量。12周后超声测量左室射血分数(LVEF)、IBS参数(包括IBS%和CVIB)；对心肌组织进行Masson染色、嗜银染色及结缔组织生长因子(CTGF)免疫组织化学染色。结果 3组间比较IBS%值SHR组>SB组(PWKY组(PSB组>WKY组(均P, 百拇医药  [关键词] 声学密度定量技术；背向散射积分；苯那普利；自发性高血压大鼠；心肌纤维化；结缔组织生长因子；

 [中图分类号] R-33；R445.1[文献标识码] A[文章编号] 1671-7562(2008)03-0245-05

 Study of the left ventricular myocardial interstitial fibrosis and effect of benazepril

 in spontaneous hypertension rats by integrated backscatter technique

 XUE Rui-feng1,SUN Shao-xian1, SUN Dong-mei1,SONG Wen-jing1，ZHANG Shu-hua2,XIE Fang2
, 百拇医药
 (1.Department of Post-graduate,North China Coal Medical University， Tangshan 063000,China；2.Department of 

 Ultrasound Diagnosis,Afiliated Hospital of North China Coal Medical University，Tangshan 063000,China)

 Abstract: Objective To assess left ventricular myocardial interstitial fibrosisand effects of benazepril on spontaneous hypertension rat(SHR)by integrated backscatter(IBS)technique. Methods Fourteen SHRs were divided equally into two groups: intervening subject(SB)group and control subject(SHR)group. Other 7 WKYs were as the normal subjects(WKY).SB group were given 10 mg kg-1 d-1 body weight of benazepril by intragastric administrstion,and WKY group and SHR group received 10 mg kg-1 d-1 of distilled water. The animals were weighed once week to adjust for drug dosage. After 12 weeks, IBS%, CVIB, left ventricular ejection(LVEF)were determined.Masson staining and argyrophilic staining were used in the study of collagen volume fraction(CVF),perivascular collagen area(PVCA)，ratio of small coronary artery wall thickness to external diameter(T/D)and argyrophilic fibers volume fraction(APFVF)in heart tissue. The expression of CTGF was evaluated by qualitative and semiquantitative immunohistochemical staining. ResultsIBS% was greater in SHR group than SB group(P, http://www.100md.com(薛瑞凤 孙绍贤 孙冬梅 宋文静 张树华 谢 芳) 第 1 2 3 页 下一页
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