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【摘要】目的观察不同培养基对人脐带间充质干细胞(hUC-MSCs)生物学特性的影响, 优化培养方法。方法采用贴壁法从正常足月胎儿脐带中分离出间充质干细胞, 细胞贴壁后利用DMEM/F12, Mesen PRORSTM Medium这两种培养体系进行体外扩增, 绘制生长曲线, 流式细胞仪检测其表面标志。结果从人脐带中分离出的间充质干细胞为梭形, 呈平行排列生长或漩涡状生长；细胞表达CD29、CD44, 不表达CD34、CD45和HLA-DR。结论加入Mesen PRORSTM Medium培养的细胞增殖速度快, 更适合于脐带间充质干细胞的体外扩增。
【关键词】间充质干细胞；脐带；生物学特性
Influence of different mediums on biological characteristics of human umbilical cord mesenchymal stem cellBAI Shang-xing.Shenyang Sixth People's Hospital, Shenyang 110000, China
【Abstract】Objective To investigate the differences of biological characteristics of human umbilical cord mesenchymal stem cells(hUC-MSCs) in different mediums, and to optimize the purify methods. Methods Mesenchymal stem cells were separated from healthy full-termed delivery fetus using tissue-adherent culture method ......
http://www.100md.com/html/paper/1673-7555/2014/14/03.htm
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