第1页参见附件(1911KB，3页)。
[摘要] 目的：探讨双黄复方(SHMC)对兔主动脉平滑肌细胞(SMC)和人胚胎成纤维细胞(CCC-ESF)的促增殖作用。方法：采用MTT法测定不同浓度、不同时间SHMC对SMC和CCC-ESF的促增殖作用，并以流式细胞术检测SHMC对其细胞周期的影响。结果：与空白对照组比较，SHMC作用后，各组OD490显著增高，G2/M期细胞比例明显增加，但SMC的增殖与剂量无明显相关。双黄复方大剂量短时间内作用于CCC-ESF的OD490、PI显著增高，小剂量长时间作用也有类似作用。结论：双黄复方对SMC和CCC-ESF生长均有促增殖作用。
[关键词] 双黄复方；平滑肌细胞；成纤维细胞；增殖
[中图分类号] R329.2[文献标识码]A [文章编号]1673-7210(2009)05(a)-027-03
Effects of Shuanghuang Herbal Medicine Compound on the proliferation of SMC and CCC-ESF
FENG Daxing1, LI Yang2
(1.The Second People's Hospital of Baiyun District of Guangzhou City, Guangzhou 510450, China; 2.Guangdong Institute for Drug Control, Guangzhou 510180, China)
[Abstract] Objective: To study the effects of Shuanghuang Herbal Medicine Compound SHMC on the proliferation of SMC and CCC-ESF. Methods: MTT was used to determine the proliferation at different concentrations and times of SHMC, and flow cytometry technique was used to detect the effects on the cell cycle of the two kinds of cells. Results: After treated by SHMC, the OD490 of SMC in each group increased markedly and the percentage of cells at G2/M phase increased markedly compared with blank control group, but the proliferation of SMC showed no relationship with the dose of SHMC. A large dose of SHMC significantly increased the OD490 and PI of CC-ESF in short time, and low dose also showed similar effects in a long time. Conclusion: SHMC could increase the proliferation of SMC and CCC-ESF.
[Key words] Shuanghuang Herbal Medicine Compound; SMC; CCC-ESF; Proliferation
压疮是人体局部血液循环受阻引起的局部皮肤溃烂和组织坏死的常见临床护理并发症，发生率为5.1%～32.1%，因缺乏特效药物，压疮已成为临床护理的一个难题[1-2]。双黄复方由黄柏、黄芩2味药组成，根据临床观察，该制剂能够治疗深肌脓肿、久卧压疮等病症。本实验以兔主动脉平滑肌细胞(SMC)和人胚胎成纤维细胞(CCC-ESF)为研究对象，考察双黄复方对细胞增殖的影响，初步探讨双黄复方的祛腐生肌作用。
1 材料与方法
1.1 实验试剂
胰蛋白酶(Trypsin)、二甲基亚砜(DMSO)、胎牛血清、DMEM培养基干粉均为Gibco产品；四甲基偶氮唑蓝(MTT，Sigma公司)。SMC和CCC-ESF购自中国医学科学院。双黄复方(批号2000710)，本院制剂。双黄复方浓缩液的制备：取15 g粉末混悬于150 ml超纯水中，浸泡24 h后过滤，滤液于65℃水浴浓缩。取浓缩物溶于5 ml高糖DMEM培养液中，过滤除菌，置4℃冰箱保存备用。
1.2 主要仪器
流式细胞仪(BECKMAN 公司)、酶标仪(Bio-Rad公司)、细胞培养温箱等。
1.3 实验方法
1.3 ......
