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  [摘要] 目的 对火焰原子吸收光谱法测定铁、锌、钙元素进行方法学评价，了解本地区儿童体内铁、锌、钙含量元素水平。 方法 绘制标准曲线，进行精密度和回收率评价；采集儿童末梢血，用含1% HNO3的0.5%曲拉通X-100(TrionX-100)1∶200稀释检测铁、锌、钙元素含量。 结果 采用该方法批内变异系数铁元素3.7%，锌元素1.9%，钙元素1.5%，批间变异系数铁元素4.5%，锌元素2.5%，钙元素2.7%，回收率为铁元素96.4%，锌元素98.0%，钙元素96.6%。儿童缺铁的百分率为13%，缺锌的百分率为10%，缺钙的百分率为10%。 结论 火焰原子吸收光谱法测定微量元素重复性好，回收率符合要求，可直接用于全血元素含量的测定，结果满意可靠。

 [关键词] 火焰原子吸收光谱；全血；儿童；微量元素

 [中图分类号] R284.1 [文献标识码] A [文章编号] 1673-7210(2012)02(b)-0112-02

 Determination of Iron, Zinc and Calcium in whole blood of children with flame atomic absorption spectrometry

 TAN Kun WANG Shu′an HU Yan LIU Weidong WANG Jian

 Xuzhou Institute of Medical Science, Jiangsu Province, Xuzhou 221006, China

 [Abstract] Objective To evaluate the methodology of flame atomic absorption spectrometry used to determine Iron, Zinc and Calcium and to understand the Iron, Zinc and Calcium element levels of children in local. Methods The standard curve was drawn, and the precision degree and recovery rate were evaluated; the peripheral blood of children was collected, and the Iron, Zinc and Calcium elements were diluted and detected with 0.5% TrionX-100 containing 1% HNO3, with a ratio of 1∶200. Results With this method, the in-batch variation coefficient was 3.7% for Iron, 1.9% for Zinc and 1.5% for Calcium, the between-batch variation coefficient was 4.5% for Iron, 2.5% for Zinc and 2.7% for Calcium, and the recovery rate was 96.4% for Iron, 98.0% for Zinc and 96.6% for Calcium. The percentage of children with Iron deficiency was 13%, Zinc deficiency was 10% and calcium deficiency was 10%. Conclusion Flame atomic absorption spectrometry shows good reproducibility and satisfactory recovery rate in the determination of trace elements, and it can be directly used for the determination of elements in whole blood, and has satisfactory and reliable results.

 [Key words] Flame atomic absorption spectrometry; Whole blood; Children; Trace elements

 微量元素是占人体总重量的1/10 000以下，每人每日需要量在100 mg以下的元素。其含量虽微，但对机体代谢起着极其重要的作用。婴幼儿微量元素缺乏对智力、免疫功能、生长发育等有较大影响[1-2]。目前，我国婴幼儿微量元素缺乏较为普遍 ......