【摘要】目的 观察重症急性胰腺炎(SAP) 大鼠肠组织中高迁移率族蛋白B1(HMGB1)表达对肠黏膜上皮细胞紧密连接occludin蛋白表达的影响。方法 逆行胰胆管注射5%牛磺胆酸钠制作SAP模型。健康Wistar大鼠随机(随机数字法)分为对照组、SAP组、二硫代氨基甲酸吡咯烷(PDTC)处理组。测定血淀粉酶(AMY)、内毒素(LPS)及D—乳酸水平；光镜下观察胰腺和肠组织的病理变化；免疫组织化学法观察occludin分布和表达的变化；RT—PCR法检测大鼠肠组织中HMGB1的表达水平；Western blot法检测大鼠肠组织中HMGB1及occludin蛋白的表达水平。采用SPSS 13.0统计分析软件进行处理，P, 百拇医药
 【关键词】胰腺炎；肠屏障；高迁移率族蛋白B1；紧密连接

 The effects of high mobility group box—1 protein on the expression of intestinal epithelial tight junction protein occludin in murine severe acute pancreatitis LUAN Zheng—gang， GUO Ren—xuan. Department of Intensive Care Unit and Department of General Surgery， The First Affiliated Hospital of China Medical University， Shenyang 110001， China

 【Abstract】Objective To observe the effect of high mobility group box—1 protein (HMGB1) on the expression of intestinal epithelial tight junction protein occludin in murine severe acute pancreatitis (SAP). Methods Rat SAP model was estabilished by retrograde injection of 5 % sodium taurocholate into choledochopancreatic duct. Healthy wistar rats were divided randomly(random number) into three groups： control group， SAP group， pyrrolidine dithiocarbamate (PDTC) therapy group. Levels of plasm amylase， lipopolysaccharide (LPS) and D—lactate were determined. The changes of morphological damage of pancreas and intestinal tissues were observed by microscopy. The distribution and expression of occludin protein were observed by SP immunohistochemistry. The mRNA expression of HMGB1 in the intestinal mucosa was detected by reverse—transcription polymerase chain reaction (RT—PCR). The expressions of HMGB1 and occludin were determined by western blotting. One—way analysis of variance was performed with SPSS Windows 13.0 statistical analysis software， and a difference was accepted as significant if P, 百拇医药(栾正刚 郭仁宣) 第 1 2 3 4 5 页 下一页
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